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imaris 3d simulation  (Oxford Instruments)
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Oxford Instruments imaris 3d simulation
a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or <t>naked</t> <t>NPs</t> both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging <t>(3D</t> view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.
Imaris 3d Simulation, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d comsol simulation model  (COMSOL Inc)
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COMSOL Inc 3d comsol simulation model
a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or <t>naked</t> <t>NPs</t> both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging <t>(3D</t> view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.
3d Comsol Simulation Model, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d helium flow simulations  (COMSOL Inc)
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a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or <t>naked</t> <t>NPs</t> both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging <t>(3D</t> view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.
3d Helium Flow Simulations, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d finite-difference time-domain (fdtd) simulations  (ANSYS inc)
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ANSYS inc 3d finite-difference time-domain (fdtd) simulations
a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or <t>naked</t> <t>NPs</t> both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging <t>(3D</t> view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.
3d Finite Difference Time Domain (Fdtd) Simulations, supplied by ANSYS inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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simscape multibody 3d simulation environment  (MathWorks Inc)
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MathWorks Inc simscape multibody 3d simulation environment
a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or <t>naked</t> <t>NPs</t> both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging <t>(3D</t> view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.
Simscape Multibody 3d Simulation Environment, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d model in comsol 6.0 simulation software  (COMSOL Inc)
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COMSOL Inc 3d model in comsol 6.0 simulation software
a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or <t>naked</t> <t>NPs</t> both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging <t>(3D</t> view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.
3d Model In Comsol 6.0 Simulation Software, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d finite-element simulations  (COMSOL Inc)
90
COMSOL Inc 3d finite-element simulations
a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or <t>naked</t> <t>NPs</t> both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging <t>(3D</t> view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.
3d Finite Element Simulations, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d microtubule assembly simulation  (MathWorks Inc)
90
MathWorks Inc 3d microtubule assembly simulation
a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or <t>naked</t> <t>NPs</t> both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging <t>(3D</t> view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.
3d Microtubule Assembly Simulation, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or naked NPs both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging (3D view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.

Journal: Nature Communications

Article Title: Dextran-based T-cell expansion nanoparticles for manufacturing CAR T cells with augmented efficacy

doi: 10.1038/s41467-025-67868-1

Figure Lengend Snippet: a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or naked NPs both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging (3D view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.

Article Snippet: White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging (3D view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 μm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between DynabeadsTM and T-Expand.

Techniques: Imaging, Incubation, Activation Assay, Two Tailed Test